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PubMed · 8972431

TGF-beta 1 forms functionally normal bone in a segmental sheep tibial diaphyseal defect.

J P Moxham·D J Kibblewhite·M Dvorak·B Perey·A F Tencer·A G Bruce·D M Strong
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Abstract

OBJECTIVE: Transforming growth factor-beta 1 (TGF-beta 1) is a polyfunctional regulatory cytokine that has been shown to have roles in extracellular matrix interactions, soft tissue healing, and osteogenesis. This study was undertaken to determine the efficacy of TGF-beta 1 in the formation of functionally normal bone in tibial-diaphyseal defects. METHOD: Seven hundred fifty micrograms of recombinant human TGF-beta 1 was added to a guanidine-extracted demineralised bone matrix (Gu-DBM) carrier and the implants were used to fill a 2.5 cm tibial diaphyseal defect in skeletally mature female sheep. The defects were allowed to heal over a 12-week period. After sacrifice, they were analyzed using four-point bending mechanical testing. RESULTS: Implants with TGF-beta 1 showed complete bony bridging of the defect and stress-strain curves similar to the normal contralateral limb, while implants with the carrier alone failed to bridge the gap. CONCLUSIONS: These results demonstrate the ability of TGF-beta 1 to induce new bone which has structural and functional characteristics similar to normal bone.

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AnimalsCalcification, PhysiologicDiaphysesFemaleOsteogenesisPostoperative PainSheepTibiaTransforming Growth Factor betaWound HealingComparative StudyJournal Article

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J P Moxham, D J Kibblewhite, M Dvorak, B Perey, A F Tencer, A G Bruce, D M Strong. 1996. TGF-beta 1 forms functionally normal bone in a segmental sheep tibial diaphyseal defect.. https://pubmed.ncbi.nlm.nih.gov/8972431/

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