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PubMed · 8978658

Development and characterization of a biodegradable polyphosphate.

M L Renier·D H Kohn
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Abstract

A biodegradable polyphosphate polymer (Mn = 18,000, Mw/Mn = 3.2) matrix system was developed as a potential delivery vehicle for growth factors. As a model system, release of recombinant human osteogenic protein-1 (OP-1) from this polymer was evaluated. The polyphosphate was synthesized using a triethylamine catalyst in an argon environment, and characterized using elemental analysis, gel permeation chromatography (GPC), and Fourier transform infrared spectroscopy (FTIR). Degradation kinetics of the polyphosphate polymer in phosphate-buffered saline (PBS) were represented by a second-order polynomial while degradation in bovine serum was linear with time. The polymer degraded faster in PBS than in bovine serum. In vitro release of OP-1 was also faster in PBS than in serum. Release kinetics of OP-1 in PBS and serum were represented by second-order polynomials. The OP-1 release from this physically dispersed polymeric matrix may be described by several possible mechanisms: diffusion, bulk polymer degradation, ion complexation, and interactions among the protein (OP-1), polymer, proteins, and enzymes in the media. This polyphosphate may be an effective carrier for morphogens, growth factors, or other classes of bioactive molecules.

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AnimalsBenzhydryl CompoundsBiocompatible MaterialsBiodegradation, EnvironmentalBone Morphogenetic Protein 7Bone Morphogenetic ProteinsCattleHumansHydrolysisMolecular WeightPhenolsPolyphosphatesRecombinant ProteinsSpectrometry, X-Ray EmissionTransforming Growth Factor betaBisphenol A CompoundsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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M L Renier, D H Kohn. 1997. Development and characterization of a biodegradable polyphosphate.. https://doi.org/10.1002/(sici)1097-4636(199701)34%3A1%3C95%3A%3Aaid-jbm13%3E3.0.co%3B2-o

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