PubMed · 8984658
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R F Service. 1996-12-20. Tumor-killer made; how does it work?. https://doi.org/10.1126/science.274.5295.2009
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Haloarchaea with the capacity to degrade alkanes is promising to deal with petroleum pollution in hypersaline environments. However, only a limited number of haloarchaeal species are investigated, and their pathway and mechanism for alkane degradation remain unclear. In this study, Halogranum rubrum RO2-11, a haloarchaeal strain, verified the ability to degrade kerosene and hexadecane in 184 g/L NaCl, with 53% and 52% degradation rates after 9 and 4 days, respectively. Genome sequencing and gene annotation indicated that strain RO2-11 possesses a complete potential alkane-degrading pathway, of which alkane hydroxylases may include CYP450, AlmA, and LadA. Transcriptome and metabolome analyses revealed that the upregulation of related genes in TCA cycle, lysine biosynthesis, and acetylation may help improve hexadecane degradation. Additionally, an alternative degrading pathway of hexadecane based on dual-terminal β-oxidation may occur in strain RO2-11. It is likely to be the first report of alkane degradation by the genus Halogranum, which may be helpful for applications of oil-pollution bioremediation under high-salt conditions.
Many sites of environmental concern contain groundwater contaminated with nonaqueous phase liquids (NAPL). In such sites interfacial processes may affect both the equilibrium and kinetic behavior of the system. In particular, insoluble hydrocarbon partitioning and microbial biodegradation of insoluble hydrocarbon are influenced by the physicochemical and interfacial characteristics of the system. A mechanistic model describing the influence of biological surfactants on microbial biodegradation of liquid-phase insoluble hydrocarbon and subsequent reduction of nonaqueous-phase liquid hydrocarbon is presented. The model consists of six coupled differential equations which use lumped kinetic parameters to describe surfactant micelle formation and diffusion to the microbial cell, nonlinear kinetic expressions for microbial growth and degradation of insoluble hydrocarbon, kinetic spatial descriptions of the change in NAPL-phase droplet size and the organic phase volume fraction with time, as well as equilibrium partitioning expressions for hydrophobic organic contaminant partioning into the surfactant micelle. The model is validated by comparison to data obtained for hexadecane degradation in a well-mixed batch system by the biosurfactant producing microorganism Pseudomonas aeruginosa strain PG201 as well as for nonproducing mutants' growth and hexadecane biodegradation in the presence of exogenously added biosurfactant. Experimentally determined biological growth parameters, as well as physical parameters such as hydrocarbon droplet size, were applied in the kinetic model. Parameter sensitivity analysis was performed on the physical and biological parameters in the model. The parameter sensitivity analysis indicates that for the biological system examined the rate of hydrocarbon solubilization and micellar transport to the cell controls the rate at which cellular uptake and biodegradation of insoluble hydrocarbon occurs. Practical aspects relating to use of the model for support of surfactant-based bioremediation efforts are discussed.
A controlled soil microcosm study was used to evaluate the performance of selected oil biotransformation indices using samples of Nigerian crude, a blended ballast oil and No. 6 fuel oil. Biotic losses were demonstrated through loss of solvent extractable matter (SEM) and changes in class fraction distribution in weathered soil extracts relative to sterilised controls. GC-EI MS peak identification and quantification was achieved for selected (sigma) n-alkanes, the isoprenoid alkanes norpristane (iC18), pristane (iC19) and phytane (iC20), combined mono-substituted (1-, 2-, 3- and 9-) methylphenanthrenes (sigma methylphenanthrenes), combined dimethylphenanthrenes (sigma dimethylphenanthrenes) and the hopane isomers 17 alpha(H)21 beta(H)-hopane and 17 alpha(H)21 beta(H)-30-norhopane. The [sigma n-alkanes:17 alpha(H)21 beta(H)-hopane] index was most sensitive to oil biotransformation and most accurately reflected depletion of oil from contaminated soils in this study. This index was found to be the most reliable for the No. 6 fuel oil saturates, dropping from 81.9 to 18.1 over the course of the 256-day microcosm study. In terms of sensitivity, and taking into account the results of an ANOVA analysis, the biotransformation indices most sensitive to oil biotransformation were (in order of decreasing sensitivity): [sigma C14-28:17 alpha(H)21 beta(H)-hopane] >> [sigma C14-28:sigma dimethylphenanthrenes] > [C18:phytane].