gamma/delta T cell/endothelial cell interactions.

Abstract

Ruminant gamma/delta T cells exhibit unique patterns of tissue- and inflammation-specific recruitment. Studies of other cells, such as alpha/beta T cells and even neutrophils, have clearly shown that interactions with the vascular endothelium regulate the entry of these cells into tissues. The leukocyte/endothelial cell interaction is a dynamic event that occurs under considerable shear force associated with blood flow, and it involves a variety of adhesion molecules expressed by both the leukocyte and endothelium. We have begun a systematic analysis of gamma/delta T cell interactions with endothelial and other cells in novel in vitro assays that reflect blood flow to gain insight into the molecular basis of the selective recruitment of these lymphocytes to epithelial-associated tissues and sites of inflammation. We have found that bovine gamma/delta T cells in newborns predominantly use the selectin family of leukocyte and vascular adhesion proteins to interact with endothelial cells. This is in direct contrast to other lymphocytes, such as alpha/beta T cells, which predominantly interact with selectins only after conversion to a memory cell phenotype. Our analyses of gamma/delta T cell-selectin interactions will be summarized.