Micropatterning fluid lipid bilayers on solid supports.

J T Groves; N Ulman; S G Boxer

doi:10.1126/science.275.5300.651

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PubMed · 9005848

Micropatterning fluid lipid bilayers on solid supports.

J T Groves·N Ulman·S G Boxer

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Abstract

Lithographically patterned grids of photoresist, aluminum oxide, or gold on oxidized silicon substrates were used to partition supported lipid bilayers into micrometer-scale arrays of isolated fluid membrane corrals. Fluorescently labeled lipids were observed to diffuse freely within each membrane corral but were confined by the micropatterned barriers. The concentrations of fluorescent probe molecules in individual corrals were altered by selective photobleaching to create arrays of fluid membrane patches with differing compositions. Application of an electric field parallel to the surface induced steady-state concentration gradients of charged membrane components in the corrals. In addition to producing patches of membrane with continuously varying composition, these gradients provide an intrinsically parallel means of acquiring information about molecular properties such as the diffusion coefficient in individual corrals.

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Aluminum Oxide Chemical Phenomena Chemistry, Physical Diffusion Electricity Fluorescent Dyes Light Lipid Bilayers Membrane Fluidity Pattern Recognition, Automated Phosphatidylcholines Silicon Dioxide Xanthenes Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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J T Groves, N Ulman, S G Boxer. 1997-01-31. Micropatterning fluid lipid bilayers on solid supports.. https://doi.org/10.1126/science.275.5300.651

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