Sequencing double-stranded DNA by strand displacement.

D J Fu; H Köster; C L Smith; C R Cantor

doi:10.1093/nar/25.3.677

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Sequencing double-stranded DNA by strand displacement.

D J Fu·H Köster·C L Smith·C R Cantor

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Abstract

Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides. This allows dsDNA targets to be captured from mixtures and directly sequenced without subcloning, purification or denaturation.

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Base Sequence Molecular Sequence Data Sequence Analysis, DNA Journal Article

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D J Fu, H Köster, C L Smith, C R Cantor. 1997-02-01. Sequencing double-stranded DNA by strand displacement.. https://doi.org/10.1093/nar%2F25.3.677

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