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PubMed · 9016681

A method for high efficiency YAC lipofection into murine embryonic stem cells.

J T Lee·R Jaenisch
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Abstract

We describe a modified protocol for introducing yeast artificial chromosomes (YACs) into murine embryonic stem (ES) cells by lipofection. With a decreased DNA:cell ratio, increased concentration of condensing agents and altered culture conditions, this protocol reduces the requirement for YAC DNA to a few micrograms, improves the recovery of neomycin-resistant ES colonies and increases the yield of clones containing both flanking vector markers and insert. These modifications enable generation of sufficient 'intact' transgenic clones for biological analysis with a single experiment.

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AnimalsCells, CulturedChromosomes, Artificial, YeastClone CellsEmbryo, MammalianMiceStem CellsTransfectionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J T Lee, R Jaenisch. 1996-12-15. A method for high efficiency YAC lipofection into murine embryonic stem cells.. https://doi.org/10.1093/nar%2F24.24.5054

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