PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 9016765

4-Hydroxynonenal interaction with rhodopsin.

F J van Kuijk
View original source ↗Export metadata ↓

Abstract

4-Hydroxynonenal binds easily to rhodopsin and this was accompanied by a decrease in measurable sulfhydryl groups. Analysis of tryptic digests of the rhodopsin-HNE adduct by high performance liquid chromatography revealed that several peptides present in the digests of rhodopsin disappeared, whereas HNE modified peptides not originally present were found in digests of the rhodopsin-HNE adduct. Matrix assisted laser desorption time of flight mass spectrometry showed that up to ten molecules of HNE bound to rhodopsin.

Explore related subjects

AldehydesAnimalsBinding SitesCattleChromatography, High Pressure LiquidCross-Linking ReagentsDithionitrobenzoic AcidMass SpectrometryPeptide FragmentsRhodopsinSulfhydryl CompoundsTrypsinJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

F J van Kuijk. 1997-01-13. 4-Hydroxynonenal interaction with rhodopsin.. https://doi.org/10.1006/bbrc.1996.5942

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

First enantiospecific total synthesis of the important biogenetic intermediates, (+)-polyneuridine and (+)-polyneuridine aldehyde, as well as 16-epi-vellosimine and macusine A.

The first enantiospecific total synthesis of the alkaloids 16-epi-vellosimine (1), (+)-polyneuridine (2), (+)-polyneuridine aldehyde (3), and macusine A (4) is reported. The key oxidation was accomplished with the Corey-Kim reagent to provide the important biogenetic intermediates, 16-epi-vellosimine (1) and polyneuridine aldehyde (3), the latter of which is required for the conversion of the sarpagan skeleton into the ajmalan system in the biosynthesis of quebrachidine. [reaction: see text].

Aldehydes↗