PubMed · 901931
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I Karube, T Matsunaga, S Mitsuda, S Suzuki. 1977. Microbial electrode BOD sensors.. https://doi.org/10.1002/bit.260191010
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The mechanism by which phospholipids translocate (flop) across the E. coli inner membrane remains to be elucidated. We tested the hypothesis that the membrane-spanning domains of proteins catalyze phospholipid flop by their mere presence in the membrane. As a model, peptides mimicking the transmembrane stretches of proteins, with the amino acid sequence GXXL(AL)(n)XXA (with X = K, H, or W and n = 8 or 12), were incorporated in large unilamellar vesicles composed of E. coli phospholipids. Phospholipid flop was measured by assaying the increase in accessibility to dithionite of a 2,6-(7-nitro-2,1,3-benzoxadiazol-4-yl)aminocaproyl (C(6)NBD)-labeled phospholipid analogue, initially exclusively present in the inner leaflet of the vesicle membrane. Fast flop of C(6)NBD-phosphatidylglycerol (C(6)NBD-PG) was observed in vesicles in which GKKL(AL)(12)KKA was incorporated, with the apparent first-order flop rate constant (K(flop)) linearly increasing with peptide:phospholipid molar ratios, reaching a translocation half-time of approximately 10 min at a 1:250 peptide:phospholipid molar ratio at 25 degrees C. The peptides of the series GXXL(AL)(8)XXA also induced flop of C(6)NBD-PG, supporting the hypothesis that transmembrane parts of proteins mediate phospholipid translocation. In this series, K(flop) decreased in the order X = K > H > W, indicating that peptide-lipid interactions in the interfacial region of the membrane modulate the efficiency of a peptide to cause flop. For the peptides tested, flop of C(6)NBD-phosphatidylethanolamine (C(6)NBD-PE) was substantially slower than that of C(6)NBD-PG. In vesicles without peptide, flop was negligible both for C(6)NBD-PG and for C(6)NBD-PE. A model for peptide-induced flop is proposed, which takes into account the observed peptide and lipid specificity.
Iron wire drawing processes involve the use of solid lubricants made of powdered raw materials, which lead to industrial wastes after being used. These wastes, based on stearates, have a negative effect on the environment. This study deals with the environmental assessment of some lubricants before and after the wire drawing process in a Spanish factory. The parameters evaluated for this study have been total organic carbon (TOC), mobility of zinc and lead, and ecotoxicity (EC(50)). Results show that wastes have more ecotoxicity than the original lubricants due to the content of metals that lubricants pick up from the wire, as pickling, patenting and galvanising take part in the manufacture. The capture of metallic particles leads to a reduction of TOC and an increase in ecotoxicity.
Irradiation of natural water samples with natural or artificial UVR typically results in a progressive loss of color and decreased absorbance; a process often referred to as photobleaching. In a typical photobleaching experiment, samples are exposed to a relatively constant level of artificial or natural UVR. However, under most natural situations, the vertical mixing of the water within the upper mixed layer results in strong and periodic fluctuations in UV irradiance. In this paper, we present the results of an experiment in which natural lake water was exposed to solar radiation in quartz tubes that were incubated either at fixed depths or rotating within the water column. We found differences between rotating and fixed samples in (i) photobleaching, (ii) nutrient release, and (iii) subsequent use by algae and bacteria. The evidence presented in this study demonstrated that photochemical processes might be affected by vertical water motion. The reasons for such differences remain largely unknown. Although we offer a potential explanation for such differences, our proposed mechanism is based on a post-hoc analysis of the data and should be taken solely as a working hypothesis for future research.