PubMed Health⌕ Search

PubMed · 9022728

PAPNET Testing System. Technical update.

Abstract

OBJECTIVE: To extensively test the functional integrity of the PAPNET Testing System, a computer-assisted cervical smear rescreening device, to ensure performance and reliability. STUDY DESIGN: Various system and subsystem testing methodologies were used to verify that the PAPNET Scanning Station performed according to specifications. Using synthetic and biologic slides, proper operation of the system in its intended use environment was verified. An automated system test was employed to demonstrate the ability of the system to detect cytologic abnormality. Several tests were performed throughout the production process and normal operation to ensure subsystem operations. RESULTS: The testing methodologies were shown to provide accurate measurements of scanning performance. CONCLUSION: Methodical testing and implementation of technologic enhancements ensure system performance and reliability. When used with microscopy, PAPNET testing provides the means to improve the accuracy of cervical cytology.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

T J Denaro, J M Herriman, O Shapira. PAPNET Testing System. Technical update.. https://doi.org/10.1159/000332307

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

An automatic 96-well solid phase extraction and liquid chromatography-tandem mass spectrometry method for the analysis of morphine, morphine-3-glucuronide and morphine-6-glucuronide in human plasma.

A bioanalytical method using automated sample transferring, automated solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed for morphine (MOR), and its metabolites morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G) in human plasma. Samples of 0.25 ml were transferred into 96-well plate using automatic liquid handler (Multiprobe II). Automated SPE was carried out on a 96-channel programmable liquid handling workstation (Quadra 96) using a C(18) sorbent. The extract was injected onto a silica column using an aqueous-organic mobile phase. The chromatographic run time was 3.5 min per injection, with retention times of 1.5, 2.0 and 2.6 min for MOR, M6G, and M3G, respectively. The detection was by monitoring MOR at m/z 286-->152, M6G and M3G at m/z 462-->286. The deuterated internal standards were monitored at m/z 289-->152 for MOR-d(3), and m/z 465-->289 for M6G-d(3) and M3G-d(3). The standard curve range was 0.5-50 ng ml(-1) for MOR, 1.0-100 ng ml(-1) for M6G, and 10-1000 ng ml(-1) for M3G. The inter-day precision and accuracy of the quality control samples were <8% relative standard deviation (RSD) and <7% relative error (RE) for MOR, <5% RSD and <2% RE for M6G, and <2% RSD and <4% RE for M3G.

Automation↗

Automated data processing on beamline FIP (BM30A) at ESRF.

Automation of protein crystallography synchrotron beamlines is becoming necessary to face challenging structural genomics projects. In this context, a program has been developed that processes diffraction frames using popular software but analyzes statistics and makes choices the way crystallographers usually do. This program includes the classical peak search, indexing, integration, scaling and anomalous signal analysis. The result, comparable with that obtained by standard users, is rapidly available, providing the required information for a more efficient use of the beam time.

Automation↗