PubMed · 9055692
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P N Baer, V J Iacono. 1995. Why microbial identification is not enough.. https://pubmed.ncbi.nlm.nih.gov/9055692/
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The methods for assessing potential biomarkers of periodontal disease activity are considered. This is necessary because a detailed examination of all the relevant research evidence is an essential process in assessing the possible clinical usefulness of a periodontal diagnostic test system based on any one of these markers.
This paper describes the new methods of periodontal diagnosis. An outline of the problems of periodontal probing techniques is followed by a discussion of the advances made by the use of constant pressure electronic probes. We finally highlight the problems of periodontal radiographical techniques, leading on to a description of computer-aided radiographic methods--in particular, digital subtraction radiology and computer-assisted linear radiology.
To examine the role of T cell subgroups, Th1 and Th2, in the development of periodontitis, the expression of various cytokines was investigated in a mouse model of alveolar bone resorption using in situ hybridization (ISH) with digoxigenin-labeled oligonucleotides. When mice received repetitive injections with Escherichia coli lipopolysaccharide into the gingiva every 48 h, alveolar bone resorption was detectable after the fourth injection, reaching a maximum after the 13th injection. For the best performance of ISH, we first had to choose a decalcification protocol. Among various decalcification protocols, 10% EDTA (4 degrees C, 5-6 days) was the best for 28S rRNA staining. Positive cells for transcripts of interferon-gamma (Th1 product) were detected after the fourth injection, reaching a maximum after the tenth injection. A similar pattern was obtained for interleukin (IL)-10 mRNA (Th2 product) and IL-1beta, while the positive cell number reached a maximum after the 13th and 10th injections, respectively. The number of IL-4 mRNA (Th2 product)-positive cells remained low till the tenth injection, but increased thereafter. Consequently, we found that the population change from Th1 to Th2 in the inflammation site correlated with the transition from gingivitis to periodontitis, indicating differential roles of T cell subgroups in the development of periodontitis.