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A method to determine the filter matrix in four-dye fluorescence-based DNA sequencing.

W Huang·Z Yin·D R Fuhrmann·D J States·L J Thomas
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Abstract

In a previous paper (Yin et al., Electrophoresis 1996, 17, 1143-1150), an automated method for matrix determination in four-dye fluorescence-based DNA sequencing was presented. As a continuation of that work, we have developed an alternative method to estimate the matrix from raw sequence data. The method uses an iterative clustering technique to associate each 4 x 1 data vector with one column of the desired filter matrix, using Kullback's I-divergence as a distance measure. The method requires less preprocessing of the data and less computation than the approach described by Yin et al. (Electrophoresis 1996, 17, 1143-1150). An example demonstrating applicability of the proposed method to Applied Biosystems sequencer data is given.

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AlgorithmsFluorescent DyesMathematicsSequence Analysis, DNAJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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W Huang, Z Yin, D R Fuhrmann, D J States, L J Thomas. 1997. A method to determine the filter matrix in four-dye fluorescence-based DNA sequencing.. https://doi.org/10.1002/elps.1150180106

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