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Transfection techniques for producing recombinant baculoviruses.

K M Trotter·H A Wood
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Abstract

The production of recombinant baculoviruses usually employs cotransfection of insect tissue-culture cells with viral and transfer-plasmid DNAs. The preparation and storage of viral and plasmid DNAs suitable for optimal transfection of insect cells are discussed. Electroporation, calcium-phosphate, and lipofection transfection techniques are presented with a discussion of their relative advantages. The rates of recombinant virus formation are compared using viral infection/plasmid transfection protocols versus cotransfection of cells with transfer-plasmid and viral DNAs.

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AnimalsBaculoviridaeGenetic VectorsRecombination, GeneticSpodopteraTransfectionJournal Article

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K M Trotter, H A Wood. 1996. Transfection techniques for producing recombinant baculoviruses.. https://doi.org/10.1007/bf02761711

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