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PubMed · 9085173

Characterization of complement C3, C4, and factor B molecules in human bile.

K Sumiyoshi·A Andoh·Y Fujiyama·H Sakumoto·T Bamba
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Abstract

We performed molecular analysis of complement components (C3, C4, and factor B) in human bile by sodium dodecyl sulfate-polyarylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Complement C3 was detected as a molecule composed of a 115-kDa alpha-chain linked to a 70-kDa beta-chain by disulfide bonds, and C3 levels ranged from 45 to 650 micrograms/ml (n = 15). C4 was detected as a triple chain (98-kDa alpha-chain, 73-kDa beta-chain, and 33-kDa gamma-chain) molecule linked by disulfide bonds, and C4 levels ranged from 2.5 to 60 micrograms/ml. Factor B, a component of the alternative pathway, was also detected, as an intact form. Factor B levels ranged from 0.3 to 8.0 micrograms/ml. The sizes and subunit structures of complement components in human bile were compatible with those reported in human serum. The results of a hemolytic assay indicated that complement molecules in human bile were functionally active. These molecules may participate in local immune and inflammatory responses in the biliary tract.

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BileComplement C3Complement C4Complement Factor BElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayHumansImmunoblottingJournal ArticleResearch Support, Non-U.S. Gov't

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K Sumiyoshi, A Andoh, Y Fujiyama, H Sakumoto, T Bamba. 1997. Characterization of complement C3, C4, and factor B molecules in human bile.. https://doi.org/10.1007/bf02936373

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