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Reverse transcriptase-like activity in Trypanosoma cruzi.

C I González·M C Thomas·F Martín·J Alcami·C Alonso·M C López
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Abstract

The use of specific synthetic RNA homopolymers as templates and short oligonucleotides as primers has allowed evidence of the existence of a reverse transcriptase-like activity in Trypanosoma cruzi, to be revealed. The RNA:DNA products derived from this reaction are of approximately 110 nucleotides in length. The enzyme has greater affinity for poly(rA)/ oligo(dT) templates than for poly(rC)/oligo(dG) having a 20 mM Mg+2 ion requirement. The detected reverse transcriptase-like activity is not affected by aphidicolin and ddTTP but is inhibited by actinomycin D. novobiocin, rifamycin SV and AZT.

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AnimalsAphidicolinCell NucleusCytoplasmDactinomycinDideoxynucleotidesEnzyme InhibitorsMagnesiumNucleic Acid Synthesis InhibitorsPoly APoly CPoly GPoly TRNA, ProtozoanRNA-Directed DNA PolymeraseReverse Transcriptase InhibitorsRifamycinsThymine NucleotidesTranscription, GeneticTrypanosomaZidovudineJournal ArticleResearch Support, Non-U.S. Gov't

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C I González, M C Thomas, F Martín, J Alcami, C Alonso, M C López. 1997. Reverse transcriptase-like activity in Trypanosoma cruzi.. https://doi.org/10.1016/s0001-706x(96)00611-0

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