Thymocyte development in vitro: implications for studies of ageing and thymic involution.

Abstract

Functional defects that accumulate in the T cell compartment are thought to be responsible for the pronounced immunodeficiency that develops during ageing, and reduced production of T cells by the thymus as it undergoes involution has been suggested to contribute to this phenomenon. Understanding the mechanisms responsible for thymic involution requires a thorough knowledge of how thymopoiesis is regulated. Obtaining such information is dependent upon the availability of defined experimental systems that permit analysis of thymopoiesis at the cellular and molecular levels. Recent advances have been made in the development of such human and murine in vitro systems, and their analysis has the potential to identify thymic microenvironmental signals that regulate T cell production. This information should, in turn, provide a basis for understanding changes in thymopoiesis that occur during ageing. The features of these culture systems are reviewed in this article, and their potential application to the study of T cell production during ageing is discussed.