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PubMed · 9105628

Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis.

P S Nelson·S Muthini·M Vierra·L Acosta·T H Smith
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Abstract

We have developed a universal solid support, termed Rainbow Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments using three different sets of primers proved that the 3'-hydroxyl function of oligodeoxyribonucleotides synthesized from Rainbow Universal CPG was retained. This universal solid support shows promise for replacing the standard nucleoside CPG supports.

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AutomationBiotinDNA, ComplementaryElectrophoresis, Polyacrylamide GelGlassHumansLithium ChlorideOligodeoxyribonucleotidesPlacentaPolymerase Chain ReactionTemperatureTemplates, GeneticJournal Article

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P S Nelson, S Muthini, M Vierra, L Acosta, T H Smith. 1997. Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis.. https://doi.org/10.2144/97224pf01

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