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PubMed · 9108176

DNA extraction by zinc.

E Kejnovský·J Kypr
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Abstract

A fast, very simple and efficient method of DNA extraction is described which takes advantage of DNA sedimentation induced by millimolar concentrations of ZnCl2. The zinc-induced sedimentation is furthermore strongly promoted by submillimolar phosphate anion concentrations. Within <30 min, the method recovers >90% of DNA irrespective of whether a plasmid DNA or short oligonucleotides are the extracted material. The method works with plasmid DNA and oligonucleotide concentrations as low as 100 ng/ml and 10 microg/ml, respectively, without using any expensive facilities or toxic chemicals.

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BuffersChloridesDNAIndicators and ReagentsZinc CompoundsJournal ArticleResearch Support, Non-U.S. Gov't

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E Kejnovský, J Kypr. 1997-05-01. DNA extraction by zinc.. https://doi.org/10.1093/nar%2F25.9.1870

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