Feulgen type staining with Hoffmann's violet-SO2 under exposure to UV rays.

M K Dutt

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PubMed · 91084

Feulgen type staining with Hoffmann's violet-SO2 under exposure to UV rays.

M K Dutt

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Abstract

The paper contains an account of the use of Hoffmann's violet-SO2 under exposure to UV rays during staining acid-hydrolysed DNA of mammalian tissue nuclei. Preparations stained with Hoffmann's violet-SO2 without exposure to UV rays reveal extremely pale violet nuclei but when stained under the influence of UV rays show a considerably faster reaction resulting in a very much deeper staining of the nuclei. Sections after staining with this dye-reagent require n-butanol as differentiating reagent. Possible interpretation for the increase in staining ability of this dye-reagent under exposure to UV rays has been elucidated and the reason for considering the reaction as Feulgen type has been discussed.

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Adrenal Glands Animals Cell Nucleus DNA Liver Male Rats Staining and Labeling Testis Ultraviolet Rays Journal Article

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M K Dutt. 1979. Feulgen type staining with Hoffmann's violet-SO2 under exposure to UV rays.. https://pubmed.ncbi.nlm.nih.gov/91084/

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