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PubMed · 9115689

Towards amperometric immunosensor devices.

L X Tiefenauer·S Kossek·C Padeste·P Thiébaud
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Abstract

In contrast to optical immunosensors, the electrochemical detection of an immunanalytical reaction does require a labeling, but allows an easier discrimination of specific and non-specific binding. We present a concept and first results for a multivalent amperometric immunosensor system which is based on silicon technology. The capture molecule streptavidin, covalently immobilized on silica, allows the immobilization of biotinylated antigens at a defined density. A nanostructured gold electrode serving as a stable network of nanowires is expected to be beneficial for the electrochemical detection of bound ferrocene-labeled antibody molecules. The results presented focus on site-specific immobilization of streptavidin on silica and reduction of non-specific binding of proteins.

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Bacterial ProteinsBiosensing TechniquesElectrochemistryElectrodesEnzyme-Linked Immunosorbent AssayEvaluation Studies as TopicGoldSilicon DioxideStreptavidinJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

L X Tiefenauer, S Kossek, C Padeste, P Thiébaud. 1997. Towards amperometric immunosensor devices.. https://doi.org/10.1016/s0956-5663(97)85339-0

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