Mammalian cell binding and transfection mediated by surface-modified bacteriophage lambda.

I S Dunn

doi:10.1016/s0300-9084(97)84338-6

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Mammalian cell binding and transfection mediated by surface-modified bacteriophage lambda.

I S Dunn

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Abstract

Bacteriophage lambda virions whose tail tube major subunit (V) proteins are modified with a cyclizable Arg-Gly-Asp (RGD) peptide are able to promote the binding of certain mammalian cells to a solid surface. This effect was shown to be specific by peptide competition experiments, and control phage lacking the RGD peptide showed no significant cellular interaction. RGD-modified but not control phage bearing a reporter gene could transfect COS cells at a significant frequency. Phage-mediated transfection therefore benefits when the efficiency of only one step in the multi-stage uptake process is improved.

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Animals Bacteriophage lambda CHO Cells COS Cells Cricetinae Genes, Reporter Genetic Vectors Integrin beta1 Lac Operon Membrane Fusion Oligopeptides Transfection Journal Article Research Support, Non-U.S. Gov't

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I S Dunn. 1996. Mammalian cell binding and transfection mediated by surface-modified bacteriophage lambda.. https://doi.org/10.1016/s0300-9084(97)84338-6

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