PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 9117891

Microfabrication and array technologies for DNA sequencing and diagnostics.

M J O'Donnell-Maloney·D P Little
View original source ↗Export metadata ↓

Abstract

It is now possible to miniaturize numerous 'macroscale' processes and develop microfabricated devices to replace conventional equipment. Such advances have lead to the development of arrays of immobilized oligonuceotides useful for basic research, diagnostic studies, sequence analysis and a number of novel applications. Additionally, standard laboratory equipment has been redesigned on a microscale level to increase efficiency; many processes have been integrated onto one chip since miniaturization can be readily achieved.

Explore related subjects

AutomationHumansIn Situ HybridizationOligodeoxyribonucleotidesRoboticsSequence Analysis, DNAConference Proceedings

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

M J O'Donnell-Maloney, D P Little. 1996. Microfabrication and array technologies for DNA sequencing and diagnostics.. https://doi.org/10.1016/s1050-3862(96)00166-0

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2002

SNP genotyping on pooled DNAs: comparison of genotyping technologies and a semi automated method for data storage and analysis.

We have compared the accuracy, efficiency and robustness of three methods of genotyping single nucleotide polymorphisms on pooled DNAs. We conclude that (i) the frequencies of the two alleles in pools should be corrected with a factor for unequal allelic amplification, which should be estimated from the mean ratio of a set of heterozygotes (k); (ii) the repeatability of an assay is more important than pinpoint accuracy when estimating allele frequencies, and assays should therefore be optimised to increase the repeatability; and (iii) the size of a pool has a relatively small effect on the accuracy of allele frequency estimation. We therefore recommend that large pools are genotyped and replicated a minimum of four times. In addition, we describe statistical approaches to allow rigorous comparison of DNA pool results. Finally, we describe an extension to our ACeDB database that facilitates management and analysis of the data generated by association studies.

Automation↗
PubMed2002

Deferred standards, an on-line qualification, validation and system stability probe for chromatographic assay.

Specific programming of automated HPLC systems allows total on-line qualification, validation and stability monitoring using the concept of deferred standards. Setting up such a process for routine analyses in an automated HPLC system requires specific autosampler programming as well as specific monitoring software. With an autosampler, a double injection procedure is programmed, the first introducing the sample, and the second, a few minutes deferred, the deferred control standard. Two additional compounds are therefore added to the sample before and during the chromatographic process: the intemal standard for sample quantification and the deferred standard for system control. Specific methodologies are described of how to obtain classical quantitative analysis information as well as system qualification validation stability information. Experiments were performed to develop specified methodologies to monitor the quality of quantitative analysis during the life of the column by using the deferred standard concept to probe the effects of column ageing on separation characteristics.

Automation↗