Small-angle X-ray scattering from mitochondria.

C A Mannella; D F Parsons

doi:10.1016/0005-2736(77)90103-1

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PubMed · 911829

Small-angle X-ray scattering from mitochondria.

C A Mannella·D F Parsons

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Abstract

X-ray (CuKalpha) scattering curves of rat liver mitochondria are characterized by continuously decreasing intensity from 0.5 to 5 mrad and a broad maximum centered near 20 mrad. The condensed-to-orthodox morphological transition of the inner membranes of intact mitochondria causes a dramatic decrease in scattering at very small angle and a marked shift of the 20 mrad maximum to smaller angle. A similar small-angle scattering maximum is observed with inner mitochondrial membrane fractions prepared by digitonin treatment and osmotic shock/step gradient centrifugation procedures. However, the small-angle X-ray scattering curves of mitochondria after acetone treatment and osmoticlysis/sonication are essentially continuous. These characteristics of mitochondrial X-ray scattering are discussed in terms of known structural features of the organelle.

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Anaerobiosis Animals Cell Fractionation In Vitro Techniques Mitochondria, Liver Mitochondrial Swelling Rats Scattering, Radiation X-Rays Journal Article Research Support, U.S. Gov't, P.H.S.

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C A Mannella, D F Parsons. 1977-10-17. Small-angle X-ray scattering from mitochondria.. https://doi.org/10.1016/0005-2736(77)90103-1

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