PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 91239

A rapid silver impregnation method for nervous tissue: a modified protargol-peroxide technic.

G P Loots·J M Loots·J M Brown·J L Schoeman
View original source ↗Export metadata ↓

Abstract

A rapid, reliable silver impregnation method is described for nervous tissue fixed in formol-saline, Bouin or Susa. Sections are impregnated for 10-15 minutes at room temperature or 37 C in a solution containing 0.5 g Protargol-S, 0.005-0.01 g allantoin, 1 ml of 1% Cu[NO3]2, 1 ml of 1% AgNO3, and 1-2 drops of 30% H2O2 in 100 ml distilled water. Thereafter the sections are reduced in a hydroquinone-formalin solution. This is followed by gold toning and subsequent reduction and mounting. Alternatively, following the first reduction, the silver image can be intensified by placing sections in a silver-allantoin bath which is followed by reduction and mounting. This method is very reliable and selective, making it suitable for general routine and research use.

Explore related subjects

AllantoinCopperHydrogen PeroxideNerve TissueNitratesSilver NitrateSilver ProteinsStaining and LabelingJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

G P Loots, J M Loots, J M Brown, J L Schoeman. 1979. A rapid silver impregnation method for nervous tissue: a modified protargol-peroxide technic.. https://doi.org/10.3109/10520297909112642

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1979

Mechanism of uricase action.

Uricase (urate:oxygen oxidoreductase, EC 1.7.3.3) exposes a positional and steric specificity in the enzymic conversion of urate to allantoin. C-2 of urate was recovered as C-2 of allantoin. By the consecutive oxidation and hydrolysis reactions a levorotatory intermediate was formed, presumably (-)-2-oxo-4-hydroxy-4-carbohydroxy-5-ureido-imidazoline. The absorption and optical rotation dispersion spectra of the intermediate were established. In the presence of borate buffer, the intermediate was transformed to (+)-alloxanate. The decay of the former compound depends on general base and acid catalysis. RS-(+/-)-allantoin was formed by chemical decarboxylation and S-(+)-allantoin by enzymic decarboxylation.

Allantoin↗
PubMed1979

The effect of allantoin on cellular multiplication in degenerating and regenerating nerves.

Injury to a peripheral nerve is, among other things, followed by degeneration of axons and myelin, as well as by a sharp increase in the number of cells (especially Schwann cells) in the part distal to the injury. The effect of allantoin--a cell proliferant--was tested on the above-mentioned reactions in the sciatic nerve of rats. The nerves were crushed and re-exposed 7, 14, 21, 35 and 90 days after the injury, and removed for histological examination. The results obtained in a group of rats treated with allantoin were compared with those obtained in a control group of rats. The results showed that allantoin had a statistically significant effect on the cellular multiplication seen in the nerve 7 and 14 days after the injury. Myelin degeneration was also found to be more advanced in the allantoin-treated nerve preparations examined 14 and 21 days postoperatively than in the control nerve preparations.

Allantoin↗