PubMed · 91350
[A quick and inexpensive staining method for vaginal smears].
Abstract
Vaginal cytology is a commonly used method for diagnosis of sexual cycle phases in the rat, but all staining techniques are time consuming. A simple, easy and fast technique is reported here, that is based upon the use of a Lugol solution that allows precise identification of the sexual cycle day of the rat. Following this technique, samples are taken at 8:00 a.m. and a smear is made on the glass slide adding one drop of Lugol solution. This preparation is covered and seen directly under the optic microscope.
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A Salas-Valdes. 1979. [A quick and inexpensive staining method for vaginal smears].. https://pubmed.ncbi.nlm.nih.gov/91350/
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