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PubMed · 9158764

Peptidoglycan composition of vancomycin-resistant Enterococcus faecium.

B L de Jonge·D Gage·S Handwerger
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Abstract

Muropeptide composition of peptidoglycan isolated from isogenic vancomycin-resistant and sensitive Enterococcus faecium strains was analyzed by reverse-phase high-performance liquid chromatography combined with amino acid and fast atom bombardment mass spectrometric analyses. Peptidoglycan of the sensitive and resistant strains was the same and was composed of tri- and tetrapeptides stem peptide subunits with or without aspartate or asparagine substitutions on the epsilon-amino group of the lysine residue. Thus, the synthesis of lactate-terminating peptidoglycan precursors in vancomycin-resistant E. faecium did not affect the chemical composition of peptidoglycan.

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Amino AcidsAnti-Bacterial AgentsDrug Resistance, MicrobialEnterococcus faeciumGenes, BacterialLactatesMolecular WeightMuramoylpentapeptide CarboxypeptidasePeptidoglycanPlasmidsSpectrometry, Mass, Fast Atom BombardmentVancomycinJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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B L de Jonge, D Gage, S Handwerger. 1996. Peptidoglycan composition of vancomycin-resistant Enterococcus faecium.. https://doi.org/10.1089/mdr.1996.2.225

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