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PubMed · 9158949

Putative regulatory sequence in human papillomavirus type 16 E2 open reading frame.

H Kitasato·A Tardy-Kadlec·M Smahel·R Tykva·V Vonka
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Abstract

A 114 bp fragment of the human papillomavirus type 16 (HPV16) E2 open reading frame (nt. 3142-3255) containing a putative estrogen responsive element (ERE) was amplified and cloned into pBLCAT2 plasmid in both sense (p159-4) and anti-sense (p164) orientation. The plasmids were transfected into human breast-cancer cell line MCF-7 containing estrogen receptor and the cultures were kept in the presence or absence of beta-estradiol. The chloramphenicol acetyltransferase (CAT) activity was not influenced by estrogen. However, a silencer effect was observed both in cultures transfected with p159-4 and p164 plasmids. We prepared and cloned synthetic fragments containing the putative ERE and failed to prove that the palindrome in the putative ERE was responsible for the silencer activity.

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Base SequenceBreast NeoplasmsChloramphenicol O-AcetyltransferaseConsensus SequenceEstradiolFemaleGenes, ReporterHumansOpen Reading FramesPapillomaviridaePlasmidsReceptors, EstrogenRecombinant Fusion ProteinsRegulatory Sequences, Nucleic AcidTransfectionTumor Cells, CulturedJournal Article

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H Kitasato, A Tardy-Kadlec, M Smahel, R Tykva, V Vonka. 1997. Putative regulatory sequence in human papillomavirus type 16 E2 open reading frame.. https://pubmed.ncbi.nlm.nih.gov/9158949/

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