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PubMed · 9174933

Polymer-shielded dye-affinity chromatography.

B Mattiasson·Galaev IYu·N Garg
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Abstract

Polymer-shielded dye-affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water-soluble polymer such as poly(vinylpyrrolidone) or poly(vinyl alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.

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AnimalsChromatography, AffinityColoring AgentsPolymersProteinsTriazinesJournal ArticleResearch Support, Non-U.S. Gov't

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B Mattiasson, Galaev IYu, N Garg. Polymer-shielded dye-affinity chromatography.. https://doi.org/10.1002/(sici)1099-1352(199634%2F12)9%3A5%2F6%3C509%3A%3Aaid-jmr293%3E3.0.co%3B2-y

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