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Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus.

R Brückner
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Abstract

A system for high-efficiency gene replacement in Staphylococcus carnosus and Staphylococcus xylosus has been developed, that is based on temperature-sensitive Escherichia coli-Staphylococcus shuttle vectors for fragment delivery and erythromycin resistance cassettes to facilitate selection of genomic copies of disrupted genes. The approach was tested by constructing a phosphotransferase-deficient mutant of S. carnosus and an S. xylosus mutant strain unable to utilize sucrose. Allelic replacements were observed at rather high frequencies, ranging from approximately 10% for the ptsI gene in S. carnosus up to 50% for the scrB gene in S. xylosus. These differences most likely reflect the length of homology rather than strain-specific variations in recombination efficiencies. Apart from the staphylococcal species tested in this study, the system appears to be applicable in other staphylococci.

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AllelesDrug Resistance, MicrobialErythromycinGene Transfer TechniquesGenetic VectorsGlycoside HydrolasesMutagenesis, Site-DirectedPhosphoenolpyruvate Sugar Phosphotransferase SystemPhosphotransferases (Nitrogenous Group Acceptor)Staphylococcusbeta-FructofuranosidaseJournal ArticleResearch Support, Non-U.S. Gov't

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R Brückner. 1997-06-01. Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus.. https://doi.org/10.1111/j.1574-6968.1997.tb10387.x

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