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PubMed · 9198441

Bacterial leakage in endodontics: an improved method for quantification.

P M Michaïlesco·J Valcarcel·A R Grieve·B Levallois·D Lerner
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Abstract

A new method for studying leakage of root fillings using the bacterium Pseudomonas fluorescens ATCC 13525 is described. The presence of the microorganism is detected by fluorimetry and can thus be used to measure the depth of penetration from the root apex toward the crown of the tooth. This system, applied to a number of methods of root canal filling, showed that procedures involving compaction of the gutta-percha gave a more effective seal than the use of a paste sealer with uncondensed gutta-percha. There was no statistically significant difference between the leakage results from the lateral, vertical, and thermomechanical condensed techniques.

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Dental LeakageDental Pulp CavityFluorometryHumansMicroscopy, Electron, ScanningPseudomonas fluorescensRoot Canal Filling MaterialsRoot Canal ObturationStatistics, NonparametricComparative StudyJournal Article

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P M Michaïlesco, J Valcarcel, A R Grieve, B Levallois, D Lerner. 1996. Bacterial leakage in endodontics: an improved method for quantification.. https://doi.org/10.1016/s0099-2399(96)80013-6

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The aim of this study was to compare the apical leakage of roots filled with different materials using a computerized fluid filtration technique. There were 36 freshly extracted human maxillary central incisors selected. After preparation and irrigation, 3 experimental groups of 10 roots were constituted. Ten roots were filled with AH Plus and gutta-percha, 10 roots were filled with Sealapex and gutta-percha, and 10 roots were filled with Epiphany sealer and Resilon cone using a single cone technique. Three roots were used as a positive control and three roots were used as a negative control group. Evaluation of the apical leakages was performed with a computerized fluid filtration technique. According to the results, the difference between group 3 and 1 and group 3 and 2 was statistically significant (p<0.05). Although group 2 leaked the most, there was no significant difference between group 1 and 2.

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Leakage and sealer penetration in smear-free dentin after a final rinse with 95% ethanol.

Forty extracted maxillary incisors were decoronated, prepared in a crown-down fashion and randomly divided into two groups of 16 roots each. Remaining roots served as controls. Smear layer was removed with 17% EDTA followed by 5.25% NaOCl, the canals in group N were again rinsed with NaOCl before obturation with laterally compacted gutta-percha and Roth's 801 sealer. The roots in group E were rinsed with 95% ethyl alcohol instead of NaOCl for the final rinse. Leakage was determined using a fluid-flow model. Roots were cleared, split, and sealer penetration into the dentinal tubules was measured under light-microscopy. Group E demonstrated significantly greater sealer penetration (p = 0.002) and significantly less leakage (p = 0.040), than group N. Leakage could not be significantly correlated with sealer penetration (p = 0.725). Under the conditions of this study, we found that a final rinse with 95% ethyl alcohol increased sealer penetration and decreased leakage.

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