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Does poly(ADP-ribosyl)ation regulate the DNA methylation pattern?

G Zardo·M D'Erme·A Reale·R Strom·M Perilli·P Caiafa
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Abstract

The existence of a possible correlation between poly(ADP-ribosyl)ation and DNA methylation processes was investigated. In vivo and in vitro experiments were carried out on L929 mouse fibroblasts preincubated for 24 h with or without 3-aminobenzamide, a well-known inhibitor of poly(ADP-ribose) polymerase. Both experimental approaches evidenced a close relationship between these two important nuclear enzymatic mechanisms, suggesting that the poly(ADP-ribosyl)ated isoform of H1 histone and/or long and branched protein-free ADP-ribose polymers could act as protecting agents against full methylation of the CpG dinucleotides in genomic DNA.

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AnimalsCell NucleusCells, CulturedDNADNA MethylationFibroblastsHistonesMicePoly Adenosine Diphosphate RibosePoly(ADP-ribose) PolymerasesJournal ArticleResearch Support, Non-U.S. Gov't

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G Zardo, M D'Erme, A Reale, R Strom, M Perilli, P Caiafa. 1997-07-01. Does poly(ADP-ribosyl)ation regulate the DNA methylation pattern?. https://doi.org/10.1021/bi970241s

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