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PubMed · 9206929

[Flow cytometry for extensive thoracic diagnosis].

K Bernhardt
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Abstract

Immunophenotyping of cells by flow cytometry has become a routine test to diagnose pulmonary and mediastinal diseases. Peripheral blood, extravascular fluids, bronchoalveolar lavage (BAL) and suspension of single cells obtained by fine-needle aspiration can be used. Peripheral blood (MOAb for immunophenotyping of lymphocytes: CD14, CD45, CD3, CD19, CD4, CD8, CD16/56, HLA DR, CD38, CD25) is the material of choice for detection and monitoring of immunodeficiences. BAL (MOAb for immunophenotyping of lymphocytes: CD14, CD45, CD3, CD19, CD4, CD8, CD16/56, HLA DR) is used mainly for differential diagnosis of extrinsic allergic alveolitis (low CD4/CD8 ratio) and sarcoidosis (high CD4/CD8 ratio). The enumeration of alveolar macrophage subsets is an important tool to establish diagnosis of histiocytosis X (CD1a > 3%). Extravascular fluids, suspension of single cells and BAL are preferred materials for detection and classification of non-Hodgkin lymphomas (MOAb for immunophenotyping of lymphocytes: CD14, CD45, CD3, CD19, CD4, CD8, CD16/56, HLA DR, CD38, CD25, CD23, CD5, CDl1c, CD30, light chain immunoglobulins).

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Diagnosis, DifferentialFlow CytometryHodgkin DiseaseHumansImmunophenotypingLungLung DiseasesLung NeoplasmsLymphoma, Non-HodgkinMediastinal DiseasesMediastinal NeoplasmsMediastinumEnglish AbstractJournal ArticleReview

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K Bernhardt. 1997. [Flow cytometry for extensive thoracic diagnosis].. https://pubmed.ncbi.nlm.nih.gov/9206929/

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