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Immunocytochemistry controls using cell culture.

D F Kurtycz·R Logrono·M Leopando·A Slattery·S L Inhorn
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Abstract

A continuing problem in cytology laboratories is the lack of adequate control material for immunocytochemical testing. Usual control procedures involve testing paraffin-embedded control materials along with the patient specimens. These control materials are fundamentally unlike cytologic preparations. We have developed a method to make control preparations for immunocytochemical analysis using cultured anaplastic cells with known antigenic features from commercial sources. Cell lines included melanoma, rhabdomyosarcoma, T-cell leukemia, and squamous-cell carcinoma. Modified Saccomano and acetone fixation coupled with the cytospin technique enabled good-quality preparations. Cell lines were tested with antibodies for HMB-45, actin, leukocyte common antigen (LCA) and cytokeratin, which avidin-biotin immunoperoxidase and diaminobenzidine (DAB) as chromogens. Our final preparations were easily interpretable with excellent morphologic preservation of cellular detail. Cultured cells provide a superior method for preparing almost unlimited numbers of control slides for immunocytochemistry for laboratories with access to a tissue culture facility.

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HumansImmunohistochemistryTumor Cells, CulturedJournal Article

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D F Kurtycz, R Logrono, M Leopando, A Slattery, S L Inhorn. 1997. Immunocytochemistry controls using cell culture.. https://doi.org/10.1002/(sici)1097-0339(199707)17%3A1%3C74%3A%3Aaid-dc17%3E3.0.co%3B2-7

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