Complement recruitment using bispecific diabodies.

R E Kontermann; M G Wing; G Winter

doi:10.1038/nbt0797-629

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Complement recruitment using bispecific diabodies.

R E Kontermann·M G Wing·G Winter

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Abstract

We describe the engineering of antibody fragments produced in bacteria for recruitment of complement effector functions. From a phage display repertoire we isolated human antibody fragments directed against complement C1q, and linked these to lysozyme-specific antibody fragments, creating bispecific antibodies (diabodies). One diabody was able to recruit C1q, resulting in efficient lysis of lysozyme-coated sheep erythrocytes, and also induced rosette-formation of erythrocytes with human monocytes and phagocytosis after phorbol ester stimulation. These diabodies may have therapeutic applications requiring the activation of complement.

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Animals Antibodies, Bispecific Base Sequence Biotechnology Complement Activation Complement C1q Complement System Proteins Erythrocytes Hemolysis Humans In Vitro Techniques Monocytes Muramidase Oligodeoxyribonucleotides Protein Engineering Journal Article Research Support, Non-U.S. Gov't

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R E Kontermann, M G Wing, G Winter. 1997. Complement recruitment using bispecific diabodies.. https://doi.org/10.1038/nbt0797-629

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