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PubMed · 9222502

Post-SELEX combinatorial optimization of aptamers.

B E Eaton·L Gold·B J Hicke·N Janjić·F M Jucker·D P Sebesta·T M Tarasow·M C Willis·D A Zichi
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Abstract

In vitro selection techniques provide a means of isolating nucleic acid ligands for binding to particular protein targets. Although most aptamers have quite high affinities for their target proteins, it has been shown that post-SELEX modification can result in further enhancement of binding affinity, as well as other desired properties. This has led to the current development of a more systematic approach to aptamer optimization using a combinatorial screening methodology.

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Base SequenceInformation SystemsLigandsOligonucleotidesProteinsRNASensitivity and SpecificityStructure-Activity RelationshipJournal Article

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B E Eaton, L Gold, B J Hicke, N Janjić, F M Jucker, D P Sebesta, T M Tarasow, M C Willis, D A Zichi. 1997. Post-SELEX combinatorial optimization of aptamers.. https://doi.org/10.1016/s0968-0896(97)00044-8

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