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PubMed · 9228713

Citrate-dependent auto-antibody causing error in blood grouping.

S R Joshi
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Abstract

BACKGROUND AND OBJECTIVES: Errors in blood grouping are occasionally due to anomalous antibodies or extraneous materials in the reagents. We investigated the case of a volunteer blood donor who was originally grouped as AB, Rh-positive, by slide grouping. However, her washed red cells showed group B, which agreed with her reverse serum grouping. MATERIALS AND METHODS: Standard serologic techniques were employed throughout. RESULTS: Investigation revealed an unusual autoantibody in the donor's blood that preferentially agglutinated the red cells in the presence of citrate. Because the grouping reagents contained citrate, this led to a false grouping when whole blood was tested rather than washed red cells. The antibody reacted at 37 degrees C and at 4 degrees C, but failed to agglutinate red cells when 0.1 M solution of monosaccharides was added to the reaction mixture. The antibody agglutinated all red cells except 6 examples of the 'Bombay' phenotype included in the panel. The antibody was neutralized by H secretor saliva, and its activity was abolished by DTT reagent, indicating its possible IgM nature. CONCLUSION: These findings suggest that this anomalous antibody has anti-H specificity.

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ABO Blood-Group SystemArtifactsAutoantibodiesBlood DonorsBlood Grouping and CrossmatchingBlood Specimen CollectionCitratesFalse Positive ReactionsFemaleFucoseHemagglutininsHumansImmunoglobulin MMiddle AgedMonosaccharidesSodium CitrateCase ReportsJournal Article

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BibTeXRIS

S R Joshi. 1997. Citrate-dependent auto-antibody causing error in blood grouping.. https://doi.org/10.1046/j.1423-0410.1997.7240229.x

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