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Decrease of rotenone inhibition is a sensitive parameter of complex I damage in brain non-synaptic mitochondria of aged rats.

M L Genova·C Bovina·M Marchetti·F Pallotti·C Tietz·G Biagini·A Pugnaloni·C Viticchi·A Gorini·R F Villa·G Lenaz
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Abstract

We investigated NADH oxidation in non-synaptic and synaptic mitochondria from brain cortex of 4- and 24-month-old rats. The NADH oxidase activity was significantly lower in non-synaptic mitochondria from aged rats; we also found a significant decrease of sensitivity of NADH oxidation to the specific Complex I inhibitor, rotenone. Since the rotenone-binding site encompasses Complex I subunits encoded by mtDNA, these results are in accordance with the mitochondrial theory of aging, whereby somatic mtDNA mutations are at the basis of cellular senescence. Accordingly, a 5 kb deletion was detected only in the cortex of the aged animals.

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AgingAnimalsCerebral CortexDNA, MitochondrialEnzyme InhibitorsMaleMitochondriaMultienzyme ComplexesNADNAD(P)H Dehydrogenase (Quinone)NADH, NADPH OxidoreductasesNeurogliaNeuronsRatsRats, WistarRotenoneSynapsesJournal ArticleResearch Support, Non-U.S. Gov't

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M L Genova, C Bovina, M Marchetti, F Pallotti, C Tietz, G Biagini, A Pugnaloni, C Viticchi, A Gorini, R F Villa, G Lenaz. 1997-06-30. Decrease of rotenone inhibition is a sensitive parameter of complex I damage in brain non-synaptic mitochondria of aged rats.. https://doi.org/10.1016/s0014-5793(97)00638-8

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