Characterizing human colorectal carcinomas by proteolytic profile.

M J Murnane; S Shuja; E Del Re; J Cai; C Iacobuzio-Donahue; V Klepeis

PubMed · 9239513

Characterizing human colorectal carcinomas by proteolytic profile.

M J Murnane·S Shuja·E Del Re·J Cai·C Iacobuzio-Donahue·V Klepeis

Abstract

Clinicopathologic staging of colorectal cancers cannot always predict aggressiveness of prognosis for a particular patient. We have used activity assays for cysteine proteinases, cathepsins B, L and H (CB, CL and CH) and matrix metalloproteinases, MMP-2 and MMP-9, to identify several distinctive, reproducible proteolytic profiles in a large set of colorectal carcinomas. We observed that individual proteinases demonstrated specific and distinct levels of activity at different cancer stages, possible reflecting non-random steps in a proteolytic cascade related to tumor development. We also observed that individual colon cancers fell into relatively few categories when characterized for the combined expression of three proteinases: CB, CL and MMP-9. Four proteolytic profiles, designated "Early", "Middle", "Late", and "High", could be used to define almost 80% of the colorectal carcinomas analyzed. Such profiles, based on the expression of several proteinases in a given tumor, provided information independent of clinical stage and may identify crucial variations in tumor behavior.

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Biomarkers, Tumor Cathepsin B Cathepsin H Cathepsin L Cathepsins Collagenases Colorectal Neoplasms Cysteine Endopeptidases Endopeptidases Gelatinases Humans Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Metalloendopeptidases Neoplasm Staging Journal Article Research Support, U.S. Gov't, P.H.S.

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M J Murnane, S Shuja, E Del Re, J Cai, C Iacobuzio-Donahue, V Klepeis. Characterizing human colorectal carcinomas by proteolytic profile.. https://pubmed.ncbi.nlm.nih.gov/9239513/

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