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PubMed · 9246790

PolI-driven integrative expression vectors for yeast.

P Blancafort·G Ferbeyre·C Sariol·R Cedergren
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Abstract

A novel expression vector for yeast has been constructed from the regulatory elements present in the polI promoter and the enhancer/termination region (E/T) of rDNA. Under some conditions, this promoter/vector combination produces small RNAs such as the hammerhead RNA sequence at levels comparable to polII- and polIII-dependent systems. No stable transcription product can be demonstrated with this vector when the enhancer/termination sequence is less than 100 nucleotides downstream from the promoter. On the other hand, high expression of a stable, hammerhead RNA molecule can be obtained from this vector by inserting a 400-bp fragment containing the ADH1 transcription termination region upstream of the E/T. RNAs produced by this vector are polyadenylated and multiple copies of this plasmid can be stably integrated into the yeast chromosome.

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Genetic VectorsRNA Polymerase IRNA, MessengerYeastsJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

P Blancafort, G Ferbeyre, C Sariol, R Cedergren. 1997-07-23. PolI-driven integrative expression vectors for yeast.. https://doi.org/10.1016/s0168-1656(97)00078-3

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