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PubMed · 9257263

Water immiscible solvent based immunoassay.

J P Aston·E J Hitchings·R L Ball·I Weeks·J S Woodhead
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Abstract

A competitive monoclonal antibody-based immunoassay which quantifies a hydrophobic hapten (Rx) in water immiscible solvents, obviating the need of a pre-extraction step, has been developed. Approximately linear dose response profiles of analyte, over the range 1-20 ugml-1 in the hydrophobic solvents, hexane, toluene and xylene were obtained. UV spectrophotometric analyses of Rx dosed hexane confirm the phenomenon of antibody-mediated transfer of analyte from the organic to the aqueous milieu. Preliminary data on the effect of water immiscible solvents on the immunoreactivity of a monoclonal antibody in free solution are presented. The potential industrial applications of water immiscible solvent based immunoassays are discussed.

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Antibodies, MonoclonalDose-Response Relationship, ImmunologicEnzyme-Linked Immunosorbent AssayHaptensHumansSerum AlbuminSolubilitySolventsUltraviolet RaysWaterJournal Article

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J P Aston, E J Hitchings, R L Ball, I Weeks, J S Woodhead. 1997. Water immiscible solvent based immunoassay.. https://doi.org/10.1080/01971529708005817

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