Processing and analyzing the mouse temporal bone to identify gross, cellular and subcellular pathology.

B A Bohne; G W Harding

doi:10.1016/s0378-5955(97)00019-1

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Processing and analyzing the mouse temporal bone to identify gross, cellular and subcellular pathology.

B A Bohne·G W Harding

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Abstract

A technique has been developed for preparing the mouse temporal bone for histopathological examination: first, as a whole mount to detect any gross malformations of the bony or membranous labyrinths; second, in dissected segments to localize damage in the different sensory organs and to quantify sensory- and supporting-cell losses; and finally, in semi-thick and thin sections to identify and characterize subcellular pathology. Examples are given of the successful application of this technique to mice with very different inner-ear problems, including those with an abnormally short cochlear spiral, a defective lateral semicircular canal, abnormal otoliths over the saccular macula, an increased susceptibility to noise damage and those which lack fibroblast growth factor receptor 3.

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Animals Cochlea Hair Cells, Auditory, Inner Hair Cells, Auditory, Outer Mice Microscopy, Electron Microscopy, Phase-Contrast Mutation Nerve Fibers Plastic Embedding Saccule and Utricle Spiral Ganglion Temporal Bone Tissue Fixation Journal Article Research Support, U.S. Gov't, P.H.S.

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B A Bohne, G W Harding. 1997. Processing and analyzing the mouse temporal bone to identify gross, cellular and subcellular pathology.. https://doi.org/10.1016/s0378-5955(97)00019-1

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