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PubMed · 9259287

Human mini-chromosomes in mouse embryonal stem cells.

M H Shen·J Yang·M L Loupart·A Smith·W Brown
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Abstract

We have introduced human mini-chromosomes of 4 Mb and approximately 15 Mb in size into mouse embryonal stem cells. Although these human mini-chromosomes are stable in hamster and chicken cells, they re-arrange or segregate aberrantly in the embryonal stem cells and are rapidly lost in the absence of selection. However, one of the mini-chromosomes re-arranged, acquired mouse centromeric sequences and was then stably maintained for at least 60 population doublings in culture. This mini-chromosome, which is 4 Mb in size, is a candidate for a mouse germ line chromosome vector.

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AnimalsCell FusionCell LineCentromereEmbryo, MammalianHumansMiceStem CellsY ChromosomeJournal ArticleResearch Support, Non-U.S. Gov't

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M H Shen, J Yang, M L Loupart, A Smith, W Brown. 1997. Human mini-chromosomes in mouse embryonal stem cells.. https://doi.org/10.1093/hmg%2F6.8.1375

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