Amperometric method for quantitative determination of proteins.

E M Skobets'; A P Megalins'ka

PubMed · 929713

Amperometric method for quantitative determination of proteins.

Abstract

The amperometric method is suggested for the quantitative determination of proteins. The method is based on ability of copper to form the intracomplex compounds with proteins and hardly soluble compounds with potassium ferrocyanide. The sensitivity of the method is 10 micrograms of protein per 1 ml. Main advantages of the method are as follows: a) the accuracy of the method is higher than that of the Lawry method and is 5%; b) possibility to determine proteins at any pH; c) possibility to determine stained proteins.

Explore related subjects

Ferrocyanides Indicators and Reagents Kinetics Methods Proteins Comparative Study English Abstract Journal Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

E M Skobets', A P Megalins'ka. Amperometric method for quantitative determination of proteins.. https://pubmed.ncbi.nlm.nih.gov/929713/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2005

From cluster calculations to molecular materials: a mixed pseudopotential approach to modeling mixed-valence systems.

In this paper we present a technique for finding an appropriate parameterization of ultrasoft pseudopotentials for modeling mixed-valence materials. For the example of hexacyanometallate molecular building blocks, we show how ionic cluster calculations can be used to determine a set of parameters for the metal centers. Pseudopotentials chosen in such a way are then shown to be suitable for periodic calculations of the corresponding mixed-valence materials (e.g., Prussian Blue).

Ferrocyanides↗

PubMed2004

[The Hanssen method for measurement of single glomerular filtration rate].

The human renal function is usually obtained as the sum of filtration of 2 million glomeruli in both kidneys. Different glomerular size in the deep and superficial renal cortex led physiologists to suspect differences in both filtration rates and regulation. From 1955 to 1963, a young Norwegian physician, Odd E. Hanssen (1917-64) in the department of pathological anatomy at Rikshospitalet University hospital in Oslo developed a method for estimating single glomerular filtration rate in all layers of the renal cortex of experimental animals by determining tubular content of filtered ferrocyanide. When testing the method he also made several new observations on tubular functions. After a delay of several years, the "Hanssen method" was adopted in 17 laboratories around the world. In about 50 publications the filtration distribution was described in many species under control conditions, during varying salt intake, and during exposure to various nervous and humoral stimuli. Odd Hanssen did not live to see the widespread use of his method; he died from cerebral haemorrhage in 1964 at the age of 47.

Ferrocyanides↗

PubMed2003

Elution and decomposition of cyanide in soil contaminated with various cyanocompounds.

Standard soil samples contaminated with various standard cyanocompounds were prepared. Column elution experiments and analyses were conducted. Compounds with an easy capacity for dissociation to ions, such as KCN and potassium hexacyanoferrate(III), were found to be eluted by forming free cyanide even in fresh water. Hexacyanoferrate(II) salts, such as potassium hexacyanoferrate(II) and iron(III) hexacyanoferrate(II), were found not to be dissociated in water, but were dissociated and diffused under alkaline conditions (pH >13). Hexacyanoferrate(II) ion was found to be more easily dissociated in water with a higher pH. Column tests as above were also conducted for soil samples taken from a former paint ink factory using iron(III) hexacyanoferrate(II), cyanogen chloride, potassium cyanate, copper cyanide, as well as potassium cyanide, as raw materials. It was demonstrate that iron(III) hexacyanoferrate(II) was dissociated and eluted under alkaline conditions. The elution rate was reduced when the contaminated soil was sandwiched with standard soil layers.Further, it was found that the Fe(CN)(6)(4-) ion eluted with NaOH from hexacyanoferrate acid in soil, were easily decomposed into cyanic acid or other byproducts by UV with the addition of ozone and H(2)O(2).

Ferrocyanides↗