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Partial resistance to gD-mediated interference conferred by mutations affecting herpes simplex virus type 1 gC and gK.

P E Pertel·P G Spear
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Abstract

Cells expressing herpes simplex virus (HSV) gD can be resistant to HSV entry as a result of gD-mediated interference. HSV strains differ in sensitivity to this interference, which blocks viral penetration but not binding. Previous studies have shown that mutations or variations in virion-associated gD can confer resistance to gD-mediated interference. Here we show that HSV-1 mutants selected for enhanced ability to bind and penetrate in the presence of inhibitory concentrations of heparin were partially resistant to gD-mediated interference. The resistance was largely due to the presence of two mutations: one in gC (the major heparin-binding glycoprotein) resulting in the absence of gC expression and the other in gK resulting in a syncytial phenotype. The results imply that heparin selected for mutants with altered postbinding requirements for entry. Resistance to gD-mediated interference conferred by mutations affecting gC and gK has not been previously described.

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AnimalsCHO CellsCricetinaeGenetic VariationHeparinHerpesvirus 1, HumanHumansMutagenesisMutagenesis, Site-DirectedPolymerase Chain ReactionTumor Cells, CulturedViral Envelope ProteinsViral ProteinsVirionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P E Pertel, P G Spear. 1997. Partial resistance to gD-mediated interference conferred by mutations affecting herpes simplex virus type 1 gC and gK.. https://doi.org/10.1128/jvi.71.10.8024-8028.1997

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