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Essential lymphocyte function associated 1 (LFA-1): intercellular adhesion molecule interactions for T cell-mediated B cell apoptosis by Fas/APO-1/CD95.

J Wang·M J Lenardo
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Abstract

B cells are susceptible to Fas ligand (FasL)+ CD4(+) Th1 cell-mediated apoptosis. We demonstrate that blocking the interactions between lymphocyte function associated (LFA)-1 and intercellular adhesion molecule(ICAM)-1 and ICAM-2 completely suppresses Fas-dependent B cell lysis. Antibodies to CD2 and CD48 partially suppress B cell apoptosis, whereas anti-B7.1 and anti-B7.2 antibodies have no effect. Also, B cells from ICAM-1-deficient mice are resistant to FasL+ T cell-mediated death. Our results suggest that LFA-1/ICAM interactions are crucial for Th1 cell-mediated B cell apoptosis and may contribute to the maintenance of B cell homeostasis in vivo.

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Amino Acid SequenceAnimalsAntibodiesAntigens, CDApoptosisB-LymphocytesCell Adhesion MoleculesCell LineChromium IsotopesCytotoxicity, ImmunologicFlow CytometryIntercellular Adhesion Molecule-1Lymphocyte Function-Associated Antigen-1MiceMice, Inbred StrainsMice, KnockoutMolecular Sequence DataTh1 Cellsfas ReceptorJournal ArticleResearch Support, Non-U.S. Gov't

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J Wang, M J Lenardo. 1997-10-06. Essential lymphocyte function associated 1 (LFA-1): intercellular adhesion molecule interactions for T cell-mediated B cell apoptosis by Fas/APO-1/CD95.. https://doi.org/10.1084/jem.186.7.1171

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