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PubMed · 9315473

A function for CD2 on murine B cells?

M C Keogh·J Elliot·T Norton·R A Lake
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Abstract

CD2 is expressed on murine B cells, probably as a result of a chromosomal translocation event during speciation. There are no activating antibodies to mouse CD2, but when activated non-specifically, only T cells up-regulate their expression of CD2. We investigated the expression and function of CD2 on B cells using mice transgenic for human CD2 under the control of a modified version of the autologous promoter/enhancer that included the immunoglobulin enhancer. This construction directs expression of human CD2 to the B cell compartment as well as to the T cell compartment. In this paper, we confirm that activating pairs of anti-CD2 monoclonal antibodies are mitogenic for mouse T cells transgenic for human CD2. In contrast, mouse B cells that express similar amounts of human CD2 are not stimulated to proliferate by equivalent doses of these antibodies. We were also unable to show any functional consequence for these B cells as a result of CD2 ligation.

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Adjuvants, ImmunologicAnimalsAntibodies, MonoclonalB-LymphocytesCD2 AntigensCD40 AntigensCell DivisionCells, CulturedGene Expression RegulationGenes, MHC Class IIGuanosineHumansImmunoglobulin Heavy ChainsImmunoglobulin MIonomycinLipopolysaccharidesMiceMice, TransgenicPromoter Regions, GeneticT-LymphocytesThionucleosidesJournal ArticleResearch Support, Non-U.S. Gov't

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M C Keogh, J Elliot, T Norton, R A Lake. 1997. A function for CD2 on murine B cells?. https://doi.org/10.1038/icb.1997.51

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