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PubMed · 9322736

Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression.

B Sun·K Jeyaseelan·M C Chung·T S Teo
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Abstract

A cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of approx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional.

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Amino Acid SequenceAnimalsBase SequenceBlotting, WesternCloning, MolecularCross ReactionsEscherichia coliHumansLigasesMolecular Sequence DataRabbitsRecombinant ProteinsSequence Analysis, DNASequence Homology, Amino AcidTissue DistributionUbiquitin-Activating EnzymesUbiquitin-Protein LigasesJournal ArticleResearch Support, Non-U.S. Gov't

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B Sun, K Jeyaseelan, M C Chung, T S Teo. 1997-09-01. Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression.. https://doi.org/10.1016/s0378-1119(97)00154-6

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