PubMed · 9353717
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D A Wellings, E Atherton. 1997. Standard Fmoc protocols.. https://doi.org/10.1016/s0076-6879(97)89043-x
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A procedure for the determination of glycine is described. The method is based on the reaction of the analyte with finely powdered, solid copper(II) carbonate in a continuous-flow assembly. The optimum experimental conditions of pH, temperature, sample volume, flow-rate, column length and internal diameter, and the linear range of calibration, were studied. Interference from foreign substances that accompany this amino acid in pharmaceutical formulations was studied, and the method was applied to the determination of glycine.