Folding of RNA involves parallel pathways.

J Pan; D Thirumalai; S A Woodson

doi:10.1006/jmbi.1997.1311

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Folding of RNA involves parallel pathways.

J Pan·D Thirumalai·S A Woodson

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Abstract

Folding kinetics of large RNAs are just beginning to be investigated. We show that the Tetrahymena self-splicing RNA partitions into a population that rapidly reaches the native state, and a slowly folding population that is trapped in metastable misfolded structures. Transitions from the misfolded structures to the native state involve partial unfolding. The total yield of native RNA is increased by iterative annealing of the inactive population, and mildly denaturing conditions increase the rate of folding at physiological temperatures. These results provide the first evidence that an RNA can fold by multiple parallel paths.

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Animals Electrophoresis, Polyacrylamide Gel Kinetics Models, Molecular Nucleic Acid Conformation Nucleic Acid Denaturation RNA Precursors RNA Splicing RNA, Protozoan Temperature Tetrahymena Urea Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J Pan, D Thirumalai, S A Woodson. 1997-10-17. Folding of RNA involves parallel pathways.. https://doi.org/10.1006/jmbi.1997.1311

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