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Antigen structural requirements for recognition by a cyclobutane thymine dimer-specific monoclonal antibody.

Y Komatsu·T Tsujino·T Suzuki·O Nikaido·E Ohtsuka
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Abstract

A monoclonal antibody (TDM-2) specific to a UV-induced cyclobutane pyrimidine dimer (T[cis-syn]T) has previously been established; however,the immunization had used UV-irradiated calf-thymus DNA containing a heterogeneous mixture of photoproduct sites. We investigated here the structural requirements of antigen recognition by the antibody using chemically synthesized antigen analogs. TDM-2 bound with cis-syn,but not trans-syn thymine dimer,and could bind strongly with four nucleotide analogs in which the cis-syn pyrimidine dimer was located in the center. Antigen analogs containing abasic linkers at the 5'- or 3'-side of the cis-syn cyclobutane pyrimidine dimer were synthesized and tested for binding to TDM-2. The results indicated that TDM-2 recognizes not only the cyclobutane ring but also both the 5'- and 3'-side nucleosides of the cyclobutane dimer. Furthermore,it was proved that either the 5'- or 3'-side phosphate group at a cyclobutane dimer site was absolutely required for the affinity to TDM-2. The antibody showed a strong binding to single stranded DNA but indicated little binding to double stranded DNA.

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AnimalsAntibodies, MonoclonalAntibody SpecificityAntigensBase SequenceBinding SitesBinding, CompetitiveCattleDNAEnzyme-Linked Immunosorbent AssayIn Vitro TechniquesMiceMolecular StructureOligodeoxyribonucleotidesPyrimidine DimersJournal ArticleResearch Support, Non-U.S. Gov't

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Y Komatsu, T Tsujino, T Suzuki, O Nikaido, E Ohtsuka. 1997-10-01. Antigen structural requirements for recognition by a cyclobutane thymine dimer-specific monoclonal antibody.. https://doi.org/10.1093/nar%2F25.19.3889

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